Abstract
Introduction
There have been no satisfactory therapies on stabilizing and repairing ruptured plagues
nowadays, which are the fundamental causes of acute coronary syndrome (ACS) and stroke.
The aim of this study was to investigate the therapeutic potential of bone marrow
mesenchymal stem cells (MSCs) in stabilizing and repairing ruptured plaques.
Materials and Methods
28 male New Zealand rabbits were randomly divided into 2 groups after establishment
of atherosclerotic disrupted plaque model by liquid nitrogen frostbite: MSCs transplantation
group and control group. MSCs were isolated, cultured in vitro, and labeled with BrdU. BrdU-incorporated MSCs (MSCs transplantation group) or an
equal amount of IMDM medium without MSCs (control group) were transplanted into vessels
with ruptured plaque. PAI-1, MMP-9 and hs-CRP were determined by ELISA of blood 3 days and 4 weeks after transplantation. Rabbits were sacrificed 4 weeks after transplantation and plaque repair was assessed by HE and Masson's trichrome
staining. Transplanted BrdU-positive cells were identified by immunohistochemistry.
Results
Four weeks after MSCs transplantation, PAI-1, MMP-9 and hs-CRP were reduced significantly
in all experimental animals (p < 0.001). The reduction was more evident in the transplantation group than in the control
group (p < 0.01). In addition, the transplantation group showed dramatically higher numbers of
newly formed endothelial cells, collagen fibers, and proliferative BrdU-positive cells
at plaque areas.
Conclusion
This study demonstrates that allogeneic MSCs transplantation can stabilize and repair
ruptured plaques, which represents a novel approach for ACS and stroke.
Keywords
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Article info
Publication history
Published online: April 25, 2013
Accepted:
April 1,
2013
Received in revised form:
February 25,
2013
Received:
October 15,
2012
Identification
Copyright
© 2013 Elsevier Ltd. Published by Elsevier Inc. All rights reserved.
