Abstract
Extensive research on the mechanism of action and medicinal importance of curcumin
obtained from turmeric (Curcuma longa) has unfolded its potential therapeutic value against many chronic ailments. Curcuma
oil (C.oil), the highly lipophilic component from Curcuma longa has been documented for its neuroprotective efficacy against rat cerebral ischemia-reperfusion
injury; however its effect on myocardial reperfusion injury remains unexplored. In
the present study, effect of C.oil (500 mg/kg, po) was evaluated against myocardial ischemia-reperfusion induced injury in
the rat model. C.oil failed to confer protection against cardiac injury, however significant
reversal of ADP induced platelet aggregation (p<0.05) was evident in the same animals. Moreover, collagen and thrombin induced platelet
aggregation (p<0.001) as well as tyrosine phosphorylation of various proteins in activated platelets
was also suppressed. C.oil also offered significant protection against collagen-epinephrine
induced thromboembolism in mice as well as augmented total time to occlusion against
FeCl3 induced arterial thrombosis in rats. C.oil however had no effect on coagulation parameters
(TT, PT and aPTT) and exerted a mild effect on the bleeding time. Bioavailability
of C.oil, as assessed by monitoring ar-turmerone, α,β-turmerone and curlone, was 13%,
11% and 7% respectively, indicating high systemic exposure. Moreover, longer mean
residence time (MRT) of ar-turmerone (13.2 h), α,β-turmerone (11.6 h) and Curlone (14.0 h) and plasma elimination half lives in the range of 5.5 to 7.2 h correlated with single 500 mg/kg dose regimen of C.oil. In the present study, C.oil thus seems to be an efficacious
and safe anti-platelet agent which was protective against intravascular thrombosis.
Abbreviations:
p.o. (per oral), iNOS (inducible nitric oxide synthase), eNOS (endothelial nitric oxide synthase), nNOS (neuronal nitric oxide synthase), NO (nitric oxide), ADP (adenosine diphosphate), PMA (12-phorbol 13-myristate acetate), EGTA (ethylene glycol tetraacetic acid), HRP (horseradish peroxidase), CMC (carboxymethyl cellulose), TTC (2,3,5-triphenyl tetrazolium chloride), CK-MB (creatine kinase-MB), MPO (myeloperoxidase), HTA-Br (hexadecyl trimethyl ammonium bromide), PRP (platelet rich plasma), HEPES ((N-[2-hydroxyethyl]piperazine-N’-[2-ethanesulfonic acid])), SDS (sodium dodecyl sulphate), PMSF (phenyl methyl sulphonyl fluoride), BSA (bovine serum albumin), MCAo (middle cerebral artery occlusion)Keywords
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References
- Drug discovery from medicinal plants.Life Sci. 2005; 78: 431-441
- From exotic spice to modern drug?.Cell. 2007; 130: 765-768
- Curcumin, demethoxycurcumin, bisdemethoxycurcumin, tetrahydrocurcumin and turmerones differentially regulate anti-inflammatory and anti-proliferative responses through a ROS-independent mechanism.Carcinogenesis. 2007; 28: 1765-1773
- Curcumin: the Indian solid gold.Adv Exp Med Biol. 2007; 595: 1-75
- Antimicrobial efficacy of the essential oil of Curcuma longa.Indian J Med Res. 1978; 68: 864-866
- Effect of turmeric oil and turmeric oleoresin on cytogenetic damage in patients suffering from oral submucous fibrosis.Cancer Lett. 1997; 116: 265-269
- Standardization and stability studies of neuroprotective lipid soluble fraction obtained from Curcuma longa.J Pharm Biomed Anal. 2007; 44: 1079-1086
- LC–UV Assay for Simultaneous Estimation of Aromatic Turmerone, α/β-Turmerone and Curlone: Major Bisabolane Sesquiterpenes of Turmeric Oil in Rabbit Plasma for Application to Pharmacokinetic Studies.Chromatographia. 2009; 69: 1077-1082
- Herbal medicaments for the treatment of neurocerebrovascular disorders.(United States)
- Neuroprotective efficacy and therapeutic window of curcuma oil: in rat embolic stroke model.BMC Complement Altern Med. 2008; 8: 55
- Curcuma oil modulates the nitric oxide system response to cerebral ischemia/reperfusion injury.Nitric Oxide. 2008; 19: 1-11
- Scientific and therapeutic advances in antiplatelet therapy.Nat Rev Drug Discov. 2003; 2: 15-28
- Future innovations in anti-platelet therapies.Br J Pharmacol. 2008; 154: 918-939
- Cardioprotective effects of cyclosporine A in an in vivo model of myocardial ischemia and reperfusion.Acta Anaesthesiol Scand. 2007; 51: 909-913
- Time course studies on the functional evaluation of experimental chronic myocardial infarction in rats.Mol Cell Biochem. 2004; 267: 47-58
- Improved myeloperoxidase assay for quantitation of neutrophil influx in a rat model of endotoxin-induced uveitis.J Pharmacol Toxicol Meth. 1998; 39: 169-178
- Pulmonary thromboembolism-induced alterations in nitric oxide release from rat circulating neutrophils.J Pharmacol Exp Ther. 1993; 265: 1369-1373
- Baylis-Hillman reaction assisted parallel synthesis of 3, 5-disubstituted isoxazoles and their in vivo bioevaluation as antithrombotic agents.Bioorg Med Chem. 2004; 12: 2059-2077
- Physical and functional association of the Src family kinases Fyn and Lyn with the collagen receptor glycoprotein VI-Fc receptor gamma chain complex on human platelets.J Exp Med. 1998; 188: 267-276
- Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature. 1970; 227: 680-685
- Optimization of ferric chloride induced thrombosis model in rats: Effect of anti-platelet and anti-coagulant drugs.J Pharmacol Toxicol Meth. 2009;
- Platelet collagen receptors, signaling and antagonism: emerging approaches for the prevention of intravascular thrombosis.Thromb Res. 2008; 122: 786-803
- Antifungal activity of turmeric oil extracted from Curcuma longa (Zingiberaceae).J Ethnopharmacol. 1995; 49: 163-169
- Anti-thrombotic effect of curcumin.Thromb Res. 1985; 40: 413-417
- Prevention of ischaemia-induced biochemical changes by curcumin & quinidine in the cat heart.Indian J Med Res. 1995; 101: 31-35
- Involvement of neutrophils in the pathogenesis of lethal myocardial reperfusion injury.Cardiovasc Res. 2004; 61: 481-497
- Cardioprotective function of inducible nitric oxide synthase and role of nitric oxide in myocardial ischemia and preconditioning: an overview of a decade of research.J Mol Cell Cardiol. 2001; 33: 1897-1918
- High levels of platelet inhibition with abciximab despite heightened platelet activation and aggregation during thrombolysis for acute myocardial infarction: results from TIMI (thrombolysis in myocardial infarction) 14.Circulation. 2000; 101: 2690-2695
- Increased platelet aggregability in response to shear stress in acute myocardial infarction and its inhibition by combined therapy with aspirin and cilostazol after coronary intervention.Am J Cardiol. 2000; 85: 1054-1059
- Inhibitory effect of curcumin, a food spice from turmeric, on platelet-activating factor- and arachidonic acid-mediated platelet aggregation through inhibition of thromboxane formation and Ca2+ signaling.Biochem Pharmacol. 1999; 58: 1167-1172
- Curcumin inhibits GPVI-mediated platelet activation by interfering with the kinase activity of Syk and the subsequent activation of PLCgamma2.Platelets. 2010; 21: 211-220
- Curcumin, a major component of food spice turmeric (Curcuma longa) inhibits aggregation and alters eicosanoid metabolism in human blood platelets.Prostaglandins Leukot Essent Fatty Acids. 1995; 52: 223-227
- Antiplatelet property of Curcuma longa L. rhizome-derived ar-turmerone.Bioresour Technol. 2006; 97: 1372-1376
- Platelets: A Practical Approach. Oxford University Press, USA1996: 199-214
- Mouse antithrombotic assay: a simple method for the evaluation of antithrombotic agents in vivo. Potentiation of antithrombotic activity by ethyl alcohol.J Pharmacol Exp Ther. 1983; 225: 57-60
Article info
Publication history
Published online: December 09, 2010
Accepted:
November 8,
2010
Received in revised form:
October 25,
2010
Received:
May 28,
2010
Identification
Copyright
© 2010 Elsevier Ltd. Published by Elsevier Inc. All rights reserved.
