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Anti-platelet effects of Curcuma oil in experimental models of myocardial ischemia-reperfusion and thrombosis

Published:December 09, 2010DOI:https://doi.org/10.1016/j.thromres.2010.11.007

      Abstract

      Extensive research on the mechanism of action and medicinal importance of curcumin obtained from turmeric (Curcuma longa) has unfolded its potential therapeutic value against many chronic ailments. Curcuma oil (C.oil), the highly lipophilic component from Curcuma longa has been documented for its neuroprotective efficacy against rat cerebral ischemia-reperfusion injury; however its effect on myocardial reperfusion injury remains unexplored. In the present study, effect of C.oil (500 mg/kg, po) was evaluated against myocardial ischemia-reperfusion induced injury in the rat model. C.oil failed to confer protection against cardiac injury, however significant reversal of ADP induced platelet aggregation (p<0.05) was evident in the same animals. Moreover, collagen and thrombin induced platelet aggregation (p<0.001) as well as tyrosine phosphorylation of various proteins in activated platelets was also suppressed. C.oil also offered significant protection against collagen-epinephrine induced thromboembolism in mice as well as augmented total time to occlusion against FeCl3 induced arterial thrombosis in rats. C.oil however had no effect on coagulation parameters (TT, PT and aPTT) and exerted a mild effect on the bleeding time. Bioavailability of C.oil, as assessed by monitoring ar-turmerone, α,β-turmerone and curlone, was 13%, 11% and 7% respectively, indicating high systemic exposure. Moreover, longer mean residence time (MRT) of ar-turmerone (13.2 h), α,β-turmerone (11.6 h) and Curlone (14.0 h) and plasma elimination half lives in the range of 5.5 to 7.2 h correlated with single 500 mg/kg dose regimen of C.oil. In the present study, C.oil thus seems to be an efficacious and safe anti-platelet agent which was protective against intravascular thrombosis.

      Abbreviations:

      p.o. (per oral), iNOS (inducible nitric oxide synthase), eNOS (endothelial nitric oxide synthase), nNOS (neuronal nitric oxide synthase), NO (nitric oxide), ADP (adenosine diphosphate), PMA (12-phorbol 13-myristate acetate), EGTA (ethylene glycol tetraacetic acid), HRP (horseradish peroxidase), CMC (carboxymethyl cellulose), TTC (2,3,5-triphenyl tetrazolium chloride), CK-MB (creatine kinase-MB), MPO (myeloperoxidase), HTA-Br (hexadecyl trimethyl ammonium bromide), PRP (platelet rich plasma), HEPES ((N-[2-hydroxyethyl]piperazine-N’-[2-ethanesulfonic acid])), SDS (sodium dodecyl sulphate), PMSF (phenyl methyl sulphonyl fluoride), BSA (bovine serum albumin), MCAo (middle cerebral artery occlusion)

      Keywords

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