Abstract
Introduction
To investigate the time course of changes of lactic acid dehydrogenase (LDH), LDH
isoenzymes and D-dimer levels following acute pulmonary thromboembolism (PTE).
Materials and methods
Eighteen dogs were randomly divided into three groups. Acute PTE was induced by injection
of preformed blood clots into pulmonary artery through femoral vein. Thrombin and
human fibrinogen were delivered into blood clots in embolism group I. Only thrombin
was delivered into blood clots in embolism group II. The control group received normal
saline and human fibrinogen in the same manner. Series of blood samples were collected
pre-embolism and post-embolism. LDH isoenzymes proportion and D-dimer levels were
measured.
Results
At 30 min, 1 h, 2 h, 4 h, 24 h post-embolism, the plasma D-dimer levels from embolism
group I were significantly higher than pre-embolism and those from control group at
the same intervals (p<0.05). The peak appeared at 2 h post-embolism (2.336±0.326 vs. 0.016±0.013, p<0.05). At 4 h, 24 h and 48 h post-embolism, total serum LDH activity and LDH-3 proportion
from two embolism groups were significantly higher than pre-embolism (p<0.05). The peak of LDH-3 proportion in two embolism groups both appeared at 24 h post-embolism
(0.225±0.021 vs. 0.108±0.030, 0.214±0.011 vs. 0.096±0.031, respectively. p<0.05).
Conclusions
The LDH-3 and D-dimer levels were changed dynamically with a relative specificity
manner during the course of acute massive PTE. Combination the D-dimer assay with
LDH-3 may have a potential value in diagnosing acute massive PTE.
Abbreviations:
CTA (spiral CT angiography), ELISA (enzyme-linked immunosorbent assays), LDH (Lactic acid dehydrogenase), PAP (pulmonary artery pressure), PCWP (pulmonary capillary wedge pressure), PE (pulmonary embolism), PTE (pulmonary thromboembolism.)Keywords
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Article info
Publication history
Accepted:
December 4,
2006
Received in revised form:
October 18,
2006
Received:
July 10,
2006
Footnotes
☆The authors of this publication wish to make the following declaration that the first two authors would be regarded as joint First Authors. The majority of the work that was performed in this study was conducted by them at Nantong University.
Identification
Copyright
© 2007 Elsevier Ltd. Published by Elsevier Inc. All rights reserved.
