This paper is only available as a PDF. To read, Please Download here.
The interaction of platelets with surfaces coated with collagens of type III (C III) or IV (C IV) has been studied by measuring the deposition of 51-Cr-labeled platelets and by scanning electron microscopy (SEM). Experiments were performed using platelet-rich plasma (PRP) and suspensions of gel-filtered platelets (GFP). Platelets were deposited on C III mainly as surfacebound aggregates. In contrast they were deposited on C IV mainly as spread forms of individual cells. Formation of aggregates on C III was more extensive for PRP than for GFP; in contrast platelet spreading on C IV was more extensive for GFP than for PRP. The effects of an extract of the plant feverfew on platelet-collagen interactions were determined. Fever few extract inhibited the deposition of 51-Cr-labeled platelets on both C III and C IV in a dose-dependent way. Similar concentrations of extract were needed to inhibit the formation of surface-bound aggregates and to inhibit platelet spreading in both PRP and GFP.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Thrombosis Research
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- Efficay of feverfew as prophylactic treatment of migraine.Br. Med. J. 1985; 291: 569-573
- Lancet. 1985; i (Editorial.): 1084
- Extracts of feverfew inhibit granule secretion in blood platelets and polymorphonuclear leukocytes.Lancet. 1985; i: 1071-1074
- Compounds extracted from feverfew that have anti-secretory activity contain an a-methylene butyrolactone unit.J. Pharm. Pharmacol. 1986; 38: 709-712
- The effects of acetylsalicylic acid and indomethacin on rabbit platelet adherence to collagen and sub endothelium in the presence of a low or high hematocrit.Thromb. Res. 1978; 13: 971-981
- Antianginal drug trapidil (Rocornal) inhibits spreading of platelets and formation of surface-bound thrombi-like aggregates.Thromb. Res. 1983; 32: 255-266
- Platelet spreading and thrombi formation in vitro.in: Chazov E.I. Smirnov V.N. Oganov R.G. Cardiology: An International Perspective. Plenum Press, New York-London1984: 391-409
- Gel filtration: a new technique for separation of platelets from plasma.Thromb. Diath. Haemorrh. 1971; 25: 268-278
- Time-response changes in the thrombogeneity of platelets spread on collagen-coated surfaces.Thromb. Res. 1980; 20: 335-341
- Platelet adhesion and aggregation on surfaces coated with human collagens of I, III, IV and V types.Bull. Exp. Biol. Med. (Moscow). 1984; 98: 359-364
- Platelet prostanoids in interaction of platelets with collagen substrates. II. Effects of exogenous arachidonic acid, stable PG endoperoxides analogue, and aspirin.Biomed. Biochem. Acta. 1984; 43: 373-376
- Transformation and motility of human platelets.in: Details of shape change and release reaction observed by optical and electron microscopy. Statistics, 1982 Edition. J. Cell. Biol.83. 1979: 126-142
- Relationship of ADP-induced fibrinogen binding to platelet shape change and aggregation elucidated by use of colchicine and cytochalasin B.Thromb. Haemostas. 1980; 43: 58-60
- Feverfew inhibits platelet behaviour via neutralization of cellular sulfhydryl groups.J. Pharm.Pharmacol. 1987; 39: 459-465
- Diamide — a new reagent for intracellular oxidation of glutathione to the disulfide.Biochem. Biophys. Res. Commun. 1969; 37: 593-596
- Chemical modification of cytoskeletal proteins of human blood platelets by diamide.Thromb. Res. 1984; 36: 609-618
- Alteration of platelet function by diamide corresponds to changes in phospholipid content of platelet cytoskeletons.Thromb. Res. 1987; 46: 385-390
- Changes in membrane phospholipid distribution during platelet activation.Biochem. Biophys. Acta. 1983; 736: 57-96
- Effects of diamide and iodoacetamide on the expression of the glycoprotein IIb/IIIa complex on blood platelets.Biochem. Biophys. Res. Commun. 1985; 127: 822-827
Accepted: September 24, 1987
Received: March 3, 1987
© 1987 Published by Elsevier Inc.