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Abstract
Ristocetin cofactor (VIIIR:RCo) and factor VIII-related antigen (VIIIR:Ag) were measured
in anticoagulated and non-anticoagulated blood incubated at 4°C, room temperature
(RT) or 37°C for 24 hours. A marked decrease in VIIIR:RCo, to almost undetectable
levels, and a smaller decrease in VIIIR:Ag occurred when whole blood clotted at 4°C.
These changes were slight 8r absent when blood clotted at RT or 37°C. VIIIR:RC8 lost
at 4°C was not recoverable by further incubation at 37°C but the less-marked loss
of VIIIR:Ag was partially recovered. In blood which had clotted at 4°C there was a
change in the electrophoretic profile of VIIIR:Ag on crossed immunoelectrophoresis:
there was more anodal migration of the VIIIR:Ag peak, consistent with a decrease in
the mean molecular size. Further experiments showed that the decrease in VIIIR:RCo
during coagulation at 4°C preceded the decrease in fibrinogen levels. In cell-free
plasma VIIIR:RCo also decreased markedly when coagulation occurred at 4°C. The results
show that loss of VIIIR:RCo occurs when blood is allowed to clot at 4°C: this is not
due to cryoprecipitation and does not require the presence of blood cells. The data
suggest that it is probably caused by plasma proteases activated early in the coagulation
pathway.
Keywords
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References
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Article info
Publication history
Accepted:
December 10,
1984
Received:
June 20,
1984
Identification
Copyright
© 1985 Published by Elsevier Inc.
