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Paper| Volume 37, ISSUE 6, P629-638, March 15, 1985

Fibrinogen fragment D is a recognition site for release-related platelet aggregation

DOI:https://doi.org/10.1016/0049-3848(85)90192-6
Fibrinogen fragment D is a recognition site for release-related platelet aggregation
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      Abstract

      The present study probes for the segment of the fibrinogen molecule which interacts with the platelet surface upon induction of release-related aggregation. The capability of platelets affixed with fibrinogen fragment D or fibrinogen fragment E to enhance aggregation of gel-filtered platelets (GFP) was compared with that of platelets affixed with fibrinogen. It is shown that release-related aggregation induced by either A23187, 10 μM ADP or thrombin is enhanced by the addition of fixed platelets bearing covalently bound fragment D by as much as 60–70% of the augmentation obtained by platelets bearing fibrinogen. On the other hand, fixed platelets bearing covalently-bound fragment E have no effect on the aggregation of GFP. It is concluded that fragment D bears the site for the interaction of fibrinogen with activated platelets, apparently, with surface-bound thrombospondin.

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      Article info

      Publication history

      Accepted: December 13, 1984
      Received: August 15, 1984

      Identification

      DOI: https://doi.org/10.1016/0049-3848(85)90192-6

      Copyright

      © 1985 Published by Elsevier Inc.

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