Paper| Volume 37, ISSUE 6, P629-638, March 15, 1985

Fibrinogen fragment D is a recognition site for release-related platelet aggregation

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      The present study probes for the segment of the fibrinogen molecule which interacts with the platelet surface upon induction of release-related aggregation. The capability of platelets affixed with fibrinogen fragment D or fibrinogen fragment E to enhance aggregation of gel-filtered platelets (GFP) was compared with that of platelets affixed with fibrinogen. It is shown that release-related aggregation induced by either A23187, 10 μM ADP or thrombin is enhanced by the addition of fixed platelets bearing covalently bound fragment D by as much as 60–70% of the augmentation obtained by platelets bearing fibrinogen. On the other hand, fixed platelets bearing covalently-bound fragment E have no effect on the aggregation of GFP. It is concluded that fragment D bears the site for the interaction of fibrinogen with activated platelets, apparently, with surface-bound thrombospondin.


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        • Marguerie G.A.
        • Plow E.F.
        • Edgington T.S.
        Human plateletes possess an inducible and saturable receptor specific for fibrinogen.
        J. Biol. Chem. 1979; 254: 5357-5363
        • Bennet J.S.
        • Vilaire G.
        Exposure of platelet fibrinogen receptors by ADP and epinephrine.
        J. Clin. Invest. 1979; 64: 1393-1401
        • Olsson P.
        • Blomback B.
        • Lagergren H.
        • Larsson R.
        • Tomikawa M.
        Interaction of platelets with surface-bound or conjugated fibrinogen.
        Bibliiotheca Haematol. 1978; 44: 134-138
        • Peerschke E.I.
        • Zucker M.B.
        • Grant R.A.
        • Egan J.J.
        • Johnson M.M.
        Correlation between fibrinogen binding to human platelets and platelet aggregability.
        Blood. 1980; 55: 841-847
        • Niewiarowski S.
        • Budzynski A.Z.
        • Morinelli R.A.
        • Brudzynski T.M.
        • Stewart G.J.
        Exposure of fibrinogen receptor on human platelets by proteolytic enzymes.
        J. Biol. Chem. 1981; 256: 917-925
        • Niewiarowski A.
        • Budzynski Z.
        • Lipinski B.
        Significance of the intact polypeptide chains of human fibrinogen in ADP-induced platelet aggregation.
        Blood. 1977; 49: 635-644
        • Holt J.C.
        • Mahmoud M.
        • Gaffney P.J.
        The ability of fibrinogen fragments to support ADP-induced platelet aggregation.
        Thromb. Res. 1979; 16: 427-435
        • Brosstad F.
        • Thorsen L.
        • Gogstad G.
        • Sletten K.
        • Solum N.O.
        Fibrin(OGEN) degradation products: Interference with binding of fibrinogen to ADP-stimulated platelets, platelet aggregation and binding of fibrinogen to immunoprecipitated fibrinogen receptor.
        in: Personal communication, presented at the Meeting on Platelet Membranes European Thrombosis Research Organization, Theodor Kocher Institute, University of Berne1983
        • Tomikawa M.
        • Iwamoto M.
        • Soderman S.
        • Blomback B.
        Effect of fibrinogen on ADP-induced platelet aggregation.
        Thromb. Res. 1980; 19: 841-855
        • Marguerie G.A.
        • Ardaillou N.
        • Chernel G.
        • Plow E.F.
        The binding of fibrinogen to its platelet receptor.
        in: Involvement of the D domain. J. Biol. Chem.257. 1982: 11872-11875
        • Hawiger J.
        • Kloczewiak M.
        • Timmons S.
        • Strong D.
        • Doolittle R.F.
        Interaction of fibrinogen with staphylococcal clumping factor and with platelets.
        Ann. NY. Acad. Sci. 1983; 408: 521-535
        • Kloczewiak M.
        • Timmons S.
        • Lukas T.J.
        • Hawiger J.
        The carboxy terminal segment of the chain of human fibrinogen is responsible for its interaction with platelets.
        Clin. Res. 1983; 31: 534a
        • Marder V.J.
        Immunologic structure of fibrinogen and its plasmic degradation products: Theoretical and clinical consideration.
        in: Laki K. Fibrinogen. Dekker, New York1968: 339-358
        • Doolittle R.F.
        Structural aspects of the fibrinogen to fibrin conversion.
        Adv. Protein. Chem. 1973; 27: 1-109
        • Mihalyi E.
        Proteolytic fragmentation of fibrinogen.
        in: Schmidt R.M Clinical Laboratory Science Vol. III, Section I: Hematology. CRC Press, 1980: 51-60
        • Agam G.
        • Livne A.
        Passive participation of fixed platelets in aggregation facilitated by covalently-bound fibrinogen.
        Blood. 1983; 61: 186-191
        • Agam G.
        • Shohat O.
        • Livne A.
        Thrombospondin plays a role in platelet-platelet recognition during release-related aggregation.
        in: Proc. Soc. Exp. Biol. Med.1984 (In press)
        • Nussenzweig V.
        • Seligman M.
        • Pelmont J.
        • Grabar P.
        Les products de degradation du fibrinogene humain par la plasmine.
        Annales de l'Institut Pasteur. 1961; 100: 377-389
        • Marder V.J.
        • James H.I.
        • Sherry S.
        The purification of fibrinogen degradation products by Pevikon Block Electrophoresis.
        Thromb. Daith. Haemorrh. 1969; 22: 234-239
        • Agam G.
        • Livne A.
        Partial resolution and reconstitution of platelet aggregation: Role of covalently-bound fibrinogen.
        in: Oplatka A. Balaban M Biological Structures and Coupled Flows. Academic Press, New York1983: 359-362
        • Solum N.O.
        • Stormorken H.
        Influence of fibrinogen on the aggregation of washed human blood platelets induced by ADP, thrombin, collagen and adrenaline.
        Scand. J. Clin. Lab. Invest. 1965; 17: 170-182
        • McLean J.R.
        • Maxwell R.E.
        • Hertler D.
        Fibrinogen and adenosine di-phosphate-induced aggregation of platelets.
        Nature. 1964; 202: 605-606
        • Brosstad F.
        • Thorsen L.
        • Gogstad G.
        • Sletten K.
        • Solum N.O.
        Crossed radioimmunoelectrophoretic studies on binding of plasmin and CNBR-mediated fibrin(OGEN) fragments to the fibrinogen-platelet receptor (The GPIIB-IIIA-complex).
        Thromb. Haemostas. 1983; 50: 85
        • Soria J.
        • Soria C.
        • Bertrand O.
        • Samama M.
        Fibrinogen and platelet aggregation.
        in: Role of the glycopeptidic part of the fibrinopeptide B. Biochem. Biophys. Res. Commun.82. 1978: 442-450
        • Kloczewiak M.
        • Timmons S.
        • Hawiger J.
        Localization of a site interacting with human platelet receptor on caroxy-terminal segment of human fibrinogen γ chain.
        Biochem. Biophys. Res. Commun. 1982; 107: 181-187
        • Kloczewiak M.
        • Timmons S.
        • Lukas T.
        • Hawiger J.
        Platelet receptor recognition site on human fibrinogen.
        in: Synthesis and structure-function relationship of peptides corresponding to the carboxy-terminal segment of the γ chain. Biochemistry. 23. 1984: 1767-1774
        • Plow E.F.
        • Srouji A.H.
        • Meyer D.
        • Marguerie G.
        • Ginsberg M.H.
        Evidence that three adhesive proteins interact with a common recognition site on activated platelets.
        J. Biol. Chem. 1984; 259: 5388-5391
        • Nachman R.L.
        • Leung L.L.K.
        Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen.
        J. Clin. Invest. 1982; 69: 263-269
        • Bennett J.S.
        • Vilaire G.
        • Clines D.B.
        Identification of the fibrinogen receptor on human platelets by photoaffinity labeling.
        J. Biol. Chem. 1982; 257: 8049-8054
        • Gartner T.K.
        • Gerrard J.M.
        • White J.G.
        • Williams D.C.
        Fibrinogen is the receptor for the endogenous lectin of human platelets.
        Nature. 1981; 289: 688-690
        • Leung L.L.K.
        • Nachman R.L.
        Complex formation of platelet thrombospondin with fibrinogen.
        J. Clin. Invest. 1982; 70: 542-549
        • Dixit V.M.
        • Grant G.A.
        • Frazier W.A.
        • Santoro S.A.
        Isolation of the fibrinogen binding-region of platelet thrombospondin.
        Biochem. Biophys. Res. Commun. 1984; 119: 1075-1081
        • Agam G.
        • Livne A.
        Platelet-platelet recognition during aggregation: Distinct Mechanisms determined by the release reaction.
        Thromb. Haemostas. 1984; 51: 145-149