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Abstract
A simple, sensitive and specific assay for plasminogen activators is described. The
assay utilizes fluorescein-labeled fibrinogen or fibrin at low concentrations, and
enables simultaneous evaluation of the plasminogen and fibrin dependence of the reaction,
that is, discrimination of tissue-type and urokinase-type plasminogen activators,
and non-specific proteolysis. Addition of antisera verify identification of the activator
species. The assay reagent contains plasminogen and fluorescein-labeled fibrinogen,
to which is added the specimen and then then thrombin, either at the initiation or
the termination of the reaction. Supernatant fluorescence is proportional to plasminogen
activator concentration. With a four-hour incubation, 1 milliunit (14 pg) of tissue
(melanoma) plasminogen activator (TPA) or 2 milliunit (36 pg) of urokinase (UK) may
be detected.
Keywords
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References
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Article info
Publication history
Accepted:
October 9,
1984
Received:
June 22,
1984
Identification
Copyright
© 1985 Published by Elsevier Inc.
