The 8-oxoguanine glycosylase I (hOGG1) Ser326Cys variant affects the susceptibility to multi-vessel disease in Taiwan coronary artery disease patients

Wang, Chiao-Ling; Lin, Tsung-Hsien; Lin, Hsing-Yi; Sheu, Sheng-Hsiung; Yu, Ming-Lung; Hsiao, Pi-Jung; Lin, Kun-Der; Hsu, Chin; Yang, Yi-Hsin; Shin, Shyi-Jang

doi:10.1016/j.thromres.2010.06.021

« Previous Next »Thrombosis Research
Volume 126, Issue 4 , Pages 319-323, October 2010

The 8-oxoguanine glycosylase I (hOGG1) Ser³²⁶Cys variant affects the susceptibility to multi-vessel disease in Taiwan coronary artery disease patients

Chiao-Ling Wang
- Graduate Institute of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan, ROC
- Division of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Medical University Hospital, Taiwan, ROC
- Division of Cardiology Department of Internal Medicine, Kaohsiung Medical University Hospital, Taiwan, ROC
Tsung-Hsien Lin
- Graduate Institute of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan, ROC
- Division of Cardiology Department of Internal Medicine, Kaohsiung Medical University Hospital, Taiwan, ROC
Hsing-Yi Lin
- Graduate Institute of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan, ROC
- Division of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Medical University Hospital, Taiwan, ROC
- Department of Internal medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan, ROC
Sheng-Hsiung Sheu
- Graduate Institute of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan, ROC
- Division of Cardiology Department of Internal Medicine, Kaohsiung Medical University Hospital, Taiwan, ROC
Ming-Lung Yu
- Department of Internal medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan, ROC
Pi-Jung Hsiao
- Division of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Medical University Hospital, Taiwan, ROC
Kun-Der Lin
- Graduate Institute of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan, ROC
- Division of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Medical University Hospital, Taiwan, ROC
Chin Hsu
- Department of Physiology, Graduate Institute of Medicine, Kaohsiung Medical University, Taiwan, ROC
Yi-Hsin Yang
- Division of Statistical Analysis, Department of Medical Research, Kaohsiung Medical University Hospital, Taiwan, ROC
- Department of Oral Hygiene, College of Dental Medicine, Kaohsiung Medical University in Kaohsiung, Taiwan
Shyi-Jang Shin
- Graduate Institute of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Taiwan, ROC
- Division of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Medical University Hospital, Taiwan, ROC
- Corresponding author. Division of Endocrinology and Metabolism, Kaohsiung Medical University Hospital, 100 Shih-Chuan 1st Rd, Kaohsiung 80708, Taiwan, ROC. Tel.: +886 7 3121101x7376; fax: +886 7 3122810.

Received 10 March 2010; received in revised form 20 June 2010; accepted 29 June 2010. published online 28 July 2010.

Abstract

8-hydroxydeoxyguanosine, the key lesion of oxidative DNA damage, contributes to the development of coronary artery disease (CAD). In humans, 8-hydroxydeoxyguanosine is repaired by the enzyme 8-oxoguanine glycosylase I (hOGG1). We investigated the association between the hOGG1 Ser³²⁶Cys polymorphism and the presence and the severity of CAD in a Taiwan population. Genotypes of the hOGG1 Ser³²⁶Cys polymorphism were determined from 1397 participants enrolled in this study (378 CAD patients and 1019 controls). CAD severity was indicated both by number of vessels affected (single-vessel disease, SVD vs. multi-vessel disease, MVD), and by individual diffuse score. Real-time polymerase chain reaction was used to determine genotype, using allele-specific TaqMan probes. We found that presence of the hOGG1 Ser³²⁶Cys polymorphism was associated with a significantly increased risk of CAD and multi-vessel disease when assuming a dominant model of inheritance (OR: 1.52 [95%:1.082~2.133], p=0.015; OR: 2.26 [95%:1.232~4.156], p=0.007). This result was confirmed by multivariate analysis, after adjustment for age, gender, body-mass index, diabetes hypertension, hypercholesterolemia and smoking (OR: 1.78 [95%:1.127~2.806], p<0.005; OR: 2.44 [95%:1.276~4.651], p<0.001). In the present study, hOGG1 Ser³²⁶Cys polymorphism is a novel genetic marker to be independently associated with the development and severity of CAD in Taiwanese population.