Thrombosis Research
Volume 125, Issue 6 , Pages e300-e305 , June 2010

Overexpression of podocalyxin in megakaryocytes and platelets decreases the bleeding time and enhances the agonist-induced aggregation of platelets

  • Sonia Alonso-Martin

      Affiliations

    • Department of Physiopathology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28040-Madrid, Spain
  • ,
  • Adam Nowakowski

      Affiliations

    • CIBER de Enfermedades Raras (CIBERER), Madrid, Spain
  • ,
  • Susana Larrucea

      Affiliations

    • Department of Physiopathology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28040-Madrid, Spain
  • ,
  • Darío Fernández

      Affiliations

    • CIBER de Enfermedades Raras (CIBERER), Madrid, Spain
  • ,
  • Maripaz Vilar-Egea

      Affiliations

    • Department of Physiopathology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28040-Madrid, Spain
    • CIBER de Enfermedades Raras (CIBERER), Madrid, Spain
  • ,
  • Matilde S. Ayuso

      Affiliations

    • Department of Physiopathology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28040-Madrid, Spain
    • CIBER de Enfermedades Raras (CIBERER), Madrid, Spain
  • ,
  • Roberto Parrilla

      Affiliations

    • Department of Physiopathology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28040-Madrid, Spain
    • CIBER de Enfermedades Raras (CIBERER), Madrid, Spain
    • Corresponding Author InformationCorresponding author. Centro de Investigaciones Biologicas (CSIC), Ramiro de Maeztu 9, Madrid-28040, Spain. Tel.: +34 91 5349154.

Received 2 October 2009 ,Revised 5 February 2010 ,Accepted 10 February 2010.

References 

  1. Michael AF, Blau E, Vernier RL. Glomerula polyanion: Alteration in aminonucleoside nephrosis. Lab Invest. 1970;23:649–657
  2. Kerjaschki D, Sharkey DJ, Farquhar MG. Idetification and characterization of podocalyxin, the major sialoglycoprotein of the renal glomurular epithelial cells. J Cell Biol. 1984;98:1591–1596
  3. Dekan G, Farquhar MG. Sulfate contributes to the negative change of podocalyxin, the major sialoglycoprotein of the glomerular filtration slits. Proc Natl Acad Sci. 1991;88:5398–5402
  4. Caulfield JP, Reid JJ, Farquhar MG. Alteration of the glomerular epithelium in acute aminonucleoside nephrosis: Evidence for formation of occluding junctions and epithelial cell detachment. Lab Invest. 1976;34:43–59
  5. Seiler MW, Rennke MW, Venkatachalem MA, Cotran RS. Pathogenesis of polycation-induced alteration (“fusion”) of glomerular epithelium. Lab Invest. 1977;36:48–61
  6. Doyonnas R, Kershaw DB, Duhme C, Merkens H, Chelliah S, Graf T, et al. Anuria, omphalocele, and perinatal lethality in mice lacking the CD34-related protein podocalyxin. J Exp Med. 2001;194:13–27
  7. Hara T, Nakano Y, Tanaka M, Tamura K, Sekiguchi T, Minehata K, et al. Identification of podocalyxin-like protein 1 as a novel cell surface marker for hemangioblast in the murine aorta-gonad-mesonephros region. Immunity. 1999;11:567–578
  8. Miettinen A, Solin ML, Reivinen J, Juvonen E, Vaisanen R, Holthofer H. Podocalyxin in rat platelets and megakaryocytes. Am J Pathol. 1999;154:813–822
  9. McNagny KE, Pettersson I, Rossi F, Flamme I, Shevchenko A, Mann M, et al. Thrombomucin, a novel cell surface protein that defines thrombocytes and multipotent hematopoietic progenitors. J Cell Biol. 1997;138:1395–1407
  10. Kershaw DB, Thomas PE, Wharram BL, Goyal M, Wiggins JE, Whiteside CI, et al. Molecular cloning, expression, and characterization of podocalyxinlike protein1 from rabbit as a transmembrane protein of glomerular podocytes and vascular endothelium. J Biol Chem. 1995;270:29439–29446
  11. Horvat R, Hovorka A, Dekan G, Poczewski H, Kerjaschki D. Endothelial cell membranes contain podocalyxin the major sialoprotein of visceral glomerular epithelial cells. J Cell Biol. 1986;102:484–491
  12. Kerosuo L, Juvonen E, Alitalo R, Gylling M, Kerjaschki D, Miettinen A. Podocalyxin in human haematopoietic cells. Br J Haematol. 2004;124:809–818
  13. Doyonnas R, Nielsen JS, Chelliah S, Drew E, Hara T, Miyajima A, et al. Podocalyxin is a CD-34-related marker of murine hematopoietic ítem cells and embryonic erythroid cells. Blood. 2005;105:4170–4178
  14. Schopperle WM, Kershaw DB, DeWolf WC. Human embryonal carcinoma tumor antigen, Gp200/GCTM-2, is podocalyxin. Biochem Biophys Res Commun. 2003;300:285–290
  15. Somasiri A, Nielsen JS, Makretsov N, McCoy ML, Prentice L, Gilks CB, et al. Overexpression of the anti-adhesin podocalyxin is an independent predictor of breast cancer progression. Cancer Res. 2004;64:5068–5073
  16. Casey G, Neville PJ, Liu X, Plummer SJ, Cicek MS, Krumroy LM, et al. Podocalyxin variants and risk of prostate cancer and tumor aggressiveness. Hum Mol Genet. 2006;15:735–741
  17. Larrucea S, Butta N, Arias-Salgado EG, Alonso-Martin S, Ayuso MS, Parrilla R. Expression of podocalyxin enhances the adherence, migration, and intercellular communication of cells. Exp Cell Res. 2008;314:2004–2015
  18. Rodriguez RB, Butta N, Larrucea S, Alonso S, Parrilla R. Production and characterization of murine monoclonal antibodies against human podocalyxin. Tissue Antigens. 2006;68:407–417
  19. Larrucea S, Butta N, Rodriguez RB, Alonso-Martin S, Arias-Salgado EG, Ayuso MS, et al. Podocalyxin enhances the adherence of cells to platelets. Cell Mol Life Sci. 2007;64:2859–3006
  20. Grewal PK, Uchiyama S, Ditto D, Varki N, Le DT, Nizet V, et al. The Ashwell receptor mitigates the lethal coagulopathy of sepsis. Nat Med. 2008;14:648–655
  21. Ramakrishnan V, Reeves PS, DeGuzman F, Deshpande U, Ministri-Madrid K, DuBridge RB, et al. Increased thrombin responsiveness in platelets from mice lacking glycoprotein V. Proc Natl Acad Sci U S A. 1999;96:13336–13341
  22. Hoover-Plow J, Shchurin A, Hart E, Sha J, Hill AE, Singer JB, et al. Genetic background determines response to hemostasis and thrombosis. BMC Blood Disord. 2006;6:6
  23. Tsuchida-Straeten N, Ensslen S, Schafer C, Woltje M, Denecke B, Moser M, et al. Enhanced blood coagulation and fibrinolysis in mice lacking histidine-rich glycoprotein (HRG). J Thromb Haemost. 2005;3:865–872
  24. Novak EK, Hui SW, Swank RT. Platelet storage pool deficiency in mouse pigment mutations associated with seven distinct genetic loci. Blood. 1984;63:536–544
  25. Vigano G, Gaspari F, Locatelli M, Pusineri F, Bonati M, Remuzzi G. Dose-effect and pharmacokinetics of estrogens given to correct bleeding time in uremia. Kidney Int. 1988;34:853–858
  26. Fattorutto M, Tourreau-Pham S, Mazoyer E, Bonnin P, Raphaël M, Morin F, et al. Recombinant activated factor VII decreases bleeding without increasing arterial thrombosis in rabbits. Can J Anesth. 2004;51:672–679
  27. Mezzano D, Muñoz B, Pais E, Downey P, Pereira J. Fast decrease of bleeding time by tranexamic acid in uremia. Thromb Haemost. 2000;5:785
  28. Carter JW, Ready AE, Singhroy S, Duta E, Gerrard JM. The effect of exercise on bleeding time and local production of prostacyclin and thromboxane. Eur J Appl Physiol. 1989;59:1439–6319
  29. Freedman JE, Sauter R, Battinelli EM, Ault K, Knowles C, Huang PL, et al. Deficient platelet-derived nitric oxide and enhanced hemostasis in mice lacking the NOSIII gene. Circ Res. 1999;84:1416–1421
  30. Takeda T, Go WY, Orlando RA, Farquhar MG. Expression of podocalyxin inhibits cell-cell adhesion and modifies junctional properties in Madin-Darby canine kidney cells. Mol Biol Cell. 2000;11:3219–3232
  31. Mahooti S, Graesser D, Patil S, Newman P, Duncan G, Makand T, et al. PECAM-1 (CD31) Expression Modulates Bleeding Time in Vivo. Am J Pathol. 2000;157:75–81
  32. Bombeli T, Schwartz BR, Harlan JM. Adhesion of activated platelets to endothelial cells: evidence for a GPIIbIIIa-dependent bridging mechanism and novel roles for endothelial intercellular adhesion molecule 1 (ICAM-1), alphavbeta3 integrin, and GPIbalpha. J Exp Med. 1998;187:329–339

PII: S0049-3848(10)00146-5

doi: 10.1016/j.thromres.2010.02.008

Thrombosis Research
Volume 125, Issue 6 , Pages e300-e305 , June 2010