Thrombosis Research
Volume 121, Issue 3 , Pages 309-317 , 2007

CD39/NTPDase-1 activity and expression in normal leukocytes

  • E. Dianne Pulte

      Affiliations

    • Research Service, Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, United States
    • Medicine-Hematology/Oncology, Weill Cornell Medical College, New York, NY 10021, United States
    • ,
  • M. Johan Broekman

      Affiliations

    • Research Service, Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, United States
    • Medicine-Hematology/Oncology, Weill Cornell Medical College, New York, NY 10021, United States
    • ,
  • Kim E. Olson

      Affiliations

    • Medicine-Hematology/Oncology, Weill Cornell Medical College, New York, NY 10021, United States
    • ,
  • Joan H.F. Drosopoulos

      Affiliations

    • Research Service, Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, United States
    • Medicine-Hematology/Oncology, Weill Cornell Medical College, New York, NY 10021, United States
    • ,
  • Jorge R. Kizer

      Affiliations

    • Medicine and Public Health, Weill Cornell Medical College, New York, NY 10021, United States
    • ,
  • Naziba Islam

      Affiliations

    • Research Service, Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, United States
    • Medicine-Hematology/Oncology, Weill Cornell Medical College, New York, NY 10021, United States
    • ,
  • Aaron J. Marcus

      Affiliations

    • Research Service, Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, United States
    • Medicine-Hematology/Oncology, Weill Cornell Medical College, New York, NY 10021, United States
    • Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10021, United States
    • Corresponding Author InformationCorresponding author. Hematology/Oncology Section, Medical Service, VA NY Harbor HS, 423 E 23rd Street, Room 13027W, New York, NY 10010, United States. Tel.: +1 212 686 7500x3931; fax: +1 212 951 3389.

Received 7 February 2007 ,Revised 17 April 2007 ,Accepted 24 April 2007.

References 

  1. Marcus AJ, Broekman MJ, Drosopoulos JH, Islam N, Alyonycheva TN, Safier LB, et al. The endothelial cell ecto-ADPase responsible for inhibition of platelet function is CD39. J Clin Invest. 1997;99:1351–1360
  2. Kaczmarek E, Koziak K, Sevigny J, Siegel JB, Anrather J, Beaudoin AR, et al. Identification and characterization of CD39/vascular ATP diphosphohydrolase. J Biol Chem. 1996;271:33116–33122
  3. Maliszewski CR, Delespesse GJ, Schoenborn MA, Armitage RJ, Fanslow WC, Nakajima T, et al. The CD39 lymphoid cell activation antigen. Molecular cloning and structural characterization. J Immunol. 1994;153:3574–3583
  4. Kansas GS, Wood GS, Tedder TF. Expression, distribution, and biochemistry of human CD39. Role in activation-associated homotypic adhesion of lymphocytes. J Immunol. 1991;146:2235–2244
  5. Olson KE, Pulte ED, Broekman MJ, Olson AE, Drosopoulos JH, Islam N, et al. CD39/NTPDase1 variants identified in human neutrophils regulate antithrombotic activity. Blood. 2005;106(11):11–16[Ref Type: Abstract]
  6. Mizumoto N, Kumamoto T, Robson SC, Sevigny J, Matsue H, Enjyoji K, et al. CD39 is the dominant Langerhans cell-associated ecto-NTPDase: modulatory roles in inflammation and immune responsiveness. Nat Med. 2002;8:358–365
  7. Clifford EE, Martin KA, Dalal P, Thomas R, Dubyak GR. Stage-specific expression of P2Y receptors, ecto-apyrase, and ecto-5′-nucleotidase in myeloid leukocytes. Am J Physiol. 1997;273:C973–C987
  8. Damle RN, Ghiotto F, Valetto A, Albesiano E, Fais F, Yan XJ, et al. B-cell chronic lymphocytic leukemia cells express a surface membrane phenotype of activated, antigen-experienced B lymphocytes. Blood. 2002;99:4087–4093
  9. Davi F, Delecluse HJ, Guiet P, Gabarre J, Fayon A, Gentilhomme O, et al. Burkitt-like lymphomas in AIDS patients: characterization within a series of 103 human immunodeficiency virus-associated non-Hodgkin's lymphomas. Burkitt's Lymphoma Study Group. J Clin Oncol. 1998;16:3788–3795
  10. Leal DB, Streher CA, Bertoncheli CM, Carli LF, Leal CA, da Silva JE, et al. HIV infection is associated with increased NTPDase activity that correlates with CD39-positive lymphocytes. Biochim Biophys Acta. 2005;1746:129–134
  11. Pinsky DJ, Broekman MJ, Peschon JJ, Stocking KL, Fujita T, Ramasamy R, et al. Elucidation of the thromboregulatory role of CD39/ectoapyrase in the ischemic brain. J Clin Invest. 2002;109:1031–1040
  12. El-Omar MM, Islam N, Broekman MJ, Drosopoulos JH, Roa DC, Lorin JD, et al. The ratio of ADP-to ATP-ectonucleotidase activity is reduced in patients with coronary artery disease. Thromb Res. 2005;116:199–206
  13. Marcus AJ, Broekman MJ, Drosopoulos JH, Olson KE, Islam N, Pinsky DJ, et al. Role of CD39 (NTPDase-1) in thromboregulation, cerebroprotection, and cardioprotection. Semin Thromb Hemost. 2005;31:234–246
  14. Marcus AJ, Broekman MJ, Drosopoulos JH, Islam N, Alyonycheva TN, Safier LB, et al. The endothelial cell ecto-ADPase responsible for inhibition of platelet function is CD39. J Clin Invest. 1997;99:1351–1360
  15. Langston HP, Ke Y, Gewirtz AT, Dombrowski KE, Kapp JA. Secretion of IL-2 and IFN-gamma, but not IL-4, by antigen-specific T cells requires extracellular ATP. J Immunol. 2003;170:2962–2970
  16. Dombrowski KE, Ke Y, Brewer KA, Kapp JA. Ecto-ATPase: an activation marker necessary for effector cell function. Immunol Rev. 1998;161:111–118
  17. Goepfert C, Imai M, Brouard S, Csizmadia E, Kaczmarek E, Robson SC. CD39 modulates endothelial cell activation and apoptosis. Mol Med. 2000;6:591–603
  18. Chen Y, Corriden R, Inoue Y, Yip L, Hashiguchi N, Zinkernagel A, et al. ATP release guides neutrophil chemotaxis via P2Y2 and A3 receptors. Science. 2006;314:1792–1795
  19. Gayle RB, Maliszewski CR, Gimpel SD, Schoenborn MA, Caspary RG, Richards C, et al. Inhibition of platelet function by recombinant soluble ecto-ADPase/CD39. J Clin Invest. 1998;101:1851–1859
  20. Drosopoulos JH, Broekman MJ, Islam N, Maliszewski CR, Gayle RB, Marcus AJ. Site-directed mutagenesis of human endothelial cell ecto-ADPase/soluble CD39: requirement of glutamate 174 and serine 218 for enzyme activity and inhibition of platelet recruitment. Biochemistry. 2000;39:6936–6943
  21. Sembries S, Pahl H, Stilgenbauer S, Dohner H, Schriever F. Reduced expression of adhesion molecules and cell signaling receptors by chronic lymphocytic leukemia cells with 11q deletion. Blood. 1999;93:624–631
  22. Pulte ED, Olson KE, Islam N, Broekman MJ, Ballard H, Marcus AJ. Function of CD39 (NTPDase-1) in chronic lymphocytic leukemia. Blood. 2005;106(11):11–16[Ref Type: Abstract]
  23. Luthje J, Schomburg A, Ogilvie A. Demonstration of a novel ecto-enzyme on human erythrocytes, capable of degrading ADP and of inhibiting ADP-induced platelet aggregation. Eur J Biochem. 1988;175:285–289
  24. Marcus AJ, Safier LB, Hajjar KA, Ullman HL, Islam N, Broekman MJ, et al. Inhibition of platelet function by an aspirin-insensitive endothelial cell ADPase. Thromboregulation by endothelial cells. J Clin Invest. 1991;88:1690–1696
  25. Lindholm L, Ejlertsson G, Schersten B. High risk of cerebro-cardiovascular morbidity in well treated male hypertensives. A retrospective study of 40–59-year-old hypertensives in a Swedish primary care district. Acta Med Scand. 1984;216:251–259
  26. Isles CG, Walker LM, Beevers GD, Brown I, Cameron HL, Clarke J, et al. Mortality in patients of the Glasgow Blood Pressure Clinic. J Hypertens. 1986;4:141–156
  27. Lip GY. Hypertension and the prothrombotic state. J Hum Hypertens. 2000;14:687–690
  28. Baricordi OR, Ferrari D, Melchiorri L, Chiozzi P, Hanau S, Chiari E, et al. An ATP-activated channel is involved in mitogenic stimulation of human T lymphocytes. Blood. 1996;87:682–690
  29. Dwyer KM, Deaglio S, Gao W, Friedman D, Strom TB, Robson SC. CD39 and control of cellular immune responses. Purinergic Signal. 2007;3:171–180

PII: S0049-3848(07)00198-3

doi: 10.1016/j.thromres.2007.04.008

Thrombosis Research
Volume 121, Issue 3 , Pages 309-317 , 2007

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