Thrombosis Research
Volume 118, Issue 4 , Pages 509-521 , 2006

Analyzing the mechanism of Rap1 activation in platelets: Rap1 activation is related to the release reaction mediated through the collagen receptor GPVI

  • Stephanie M. Jung

      Affiliations

    • Department of Protein Biochemistry, Institute of Life Science, Kurume University, Kurume, Fukuoka, Japan 839-0864
  • ,
  • Masaaki Ohnuma

      Affiliations

    • Department of Protein Biochemistry, Institute of Life Science, Kurume University, Kurume, Fukuoka, Japan 839-0864
  • ,
  • Naohide Watanabe

      Affiliations

    • Blood Center, Division of Hematology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan 160-8582
  • ,
  • Mamiko Sonoda

      Affiliations

    • Department of Protein Biochemistry, Institute of Life Science, Kurume University, Kurume, Fukuoka, Japan 839-0864
  • ,
  • Makoto Handa

      Affiliations

    • Blood Center, Division of Hematology, Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan 160-8582
  • ,
  • Masaaki Moroi

      Affiliations

    • Department of Protein Biochemistry, Institute of Life Science, Kurume University, Kurume, Fukuoka, Japan 839-0864
    • Corresponding Author InformationCorresponding author. Tel.: +81 942 37 6315; fax: +81 942 37 6319.

Received 19 July 2005 ,Revised 5 October 2005 ,Accepted 5 November 2005.

References 

  1. Reedquist KA, Ross E, Koop EA, Wolthuis RMF, Zwartkruis FJT, van Kooyk Y, et al. The small GTPase, Rap1, mediates CD31-induced integrin adhesion. J Cell Biol. 2000;148:1151–1158
  2. Katagiri K, Hattori M, Minato N, Kinashi T. Rap1 functions as a key regulator of T-cell and antigen-presenting cell interactions and modulates T-cell responses. Mol Cell Biol. 2002;22:1001–1015
  3. de Bruyn KMT, Rangarajan S, Reedquist KA, Figdor CG, Bos JL. The small GTPase Rap1 is required for Mn2+- and antibody-induced LFA-1- and VLA-4-mediated cell adhesion. J Biol Chem. 2002;277:29468–29476
  4. Caron E, Self AJ, Hall A. The GTPase Rap1 controls functional activation of macrophage integrin αMβ2 by LPS and other inflammatory mediators. Curr Biol. 2000;10:974–978
  5. Katagiri K, Maeda A, Shimonaka M, Kinashi T. RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. Nat Immunol. 2003;4:741–748
  6. Fischer TH, Gatling MN, Lacal JC, White GCII. Rap1B, a cAMP-dependent protein kinase substrate, associates with the platelet cytoskeleton. J Biol Chem. 1990;265:19405–19408
  7. Franke B, Akkerman J-WN, Bos JL. Rapid Ca2+-mediated activation of Rap1 in human platelets. EMBO J. 1997;16:252–259
  8. Franke B, van Triest M, de Bruijn KMT, van Willigen G, Nieuwenhuis HK, Negrier C, et al. Sequential regulation of the small GTPase Rap1 in human platelets. Mol Cell Biol. 2000;20:779–785
  9. Bertoni A, Tadokoro S, Eto K, Pampori N, Parise LV, White GC, et al. Relationships between Rap1b, affinity modulation of integrin αIIbβ3, and the actin cytoskeleton. J Biol Chem. 2002;277:25715–25721
  10. Eto K, Murphy R, Kerrigan SW, Bertoni A, Stuhlmann H, Nakano T, et al. Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling. Proc Natl Acad Sci U S A. 2002;99:12819–12824
  11. Moroi M, Jung SM, Okuma M, Shinmyozu K. A patient with platelets deficient in glycoprotein VI that lack both collagen-induced aggregation and adhesion. J Clin Invest. 1989;84:1440–1445
  12. Clemetson JM, Polgar J, Magnenat E, Wells TNC, Clemetson KJ. The platelet collagen receptor glycoprotein VI is a member of the immunoglobulin superfamily closely related to FcαR and the natural killer receptors. J Biol Chem. 1999;274:29019–29024
  13. Watson SP, Asazuma N, Atkinson B, Berlanga O, Best D, Bobe R, et al. The role of ITAM- and ITIM-coupled receptors in platelet activation by collagen. Thromb Haemost. 2001;86:276–288
  14. Larson MK, Chen H, Kahn ML, Taylor AM, Fabre J-E, Mortensen RM, et al. Identification of P2Y12-dependent and -independent mechanisms of glycoprotein VI-mediated Rap1 activation in platelets. Blood. 2003;101:1409–1415
  15. Woulfe D, Jiang H, Mortensen R, Yang J, Brass LF. Activation of Rap1B by Gi family members in platelets. J Biol Chem. 2002;277:23382–23390
  16. Hofer F, Fields S, Schneider C, Martin GS. Activated Ras interacts with the Ral guanine nucleotide dissociation stimulator. Proc Natl Acad Sci U S A. 1994;91:11089–11093
  17. Jung SM, Moroi M. Platelets interact with soluble and insoluble collagens through characteristically different reactions. J Biol Chem. 1998;273:14827–14837
  18. Morton LF, Hargreaves PG, Farndale RW, Young RD, Barnes MJ. Integrin α2β1-independent activation of platelets by simple collagen-like peptides: collagen tertiary (triple-helical) and quaternary (polymeric) structures are sufficient alone for α2β1-independent platelet reactivity. Biochem J. 1995;306:337–344
  19. Francischetti IMB, Saliou B, Leduc M, Carlini CR, Hatmi M, Randon J, et al. Convulxin, a potent platelet-aggregating protein from Crotalus durissus terrificus venom, specifically binds to platelets. Toxicom. 1997;35:1217–1228
  20. Jung SM, Moroi M. Signal-transducing mechanisms involved in activation of the platelet collagen receptor integrin α2β1. J Biol Chem. 2000;275:8016–8026
  21. Moroi M, Mizuguchi J, Kawashima S, Nagamatsu M, Miura Y, Nakagaki T, et al. A new monoclonal antibody, mAb 204-11, that influences the binding of platelet GPVI to fibrous collagen. Thromb Haemost. 2003;89:996–1003
  22. McNicol A. Platelet preparation and estimation of functional responses. In:  Watson SP,  Authi KS editor. Platelets. Oxford: IRL Press; 1996;p. 1–26
  23. Suzuki H, Terauchi Y, Fujiwara M, Aizawa S, Yazaki Y, Kadowaki T, et al. Xid-like immunodeficiency in mice with disruption of the p85alpha subunit of phosphoinositide 3-kinase. Science. 1999;283:390–392
  24. Watanabe N, Nakajima H, Suzuki H, Oda A, Matsubara Y, Moroi M, et al. Functional phenotype of phosphoinositide 3-kinase p85α-null platelets characterized by an impaired response to GP VI stimulation. Blood. 2003;102:541–548
  25. Jung SM, Moroi M. Platelet collagen receptor integrin α2β1 activation involves differential participation of ADP-receptor subtypes P2Y1 and P2Y12 but not intracellular calcium change. Eur J Biochem. 2001;268:3513–3522
  26. Lova P, Paganini S, Hirsch E, Barberis L, Wymann M, Sinigaglia F, et al. A selective role for phosphatidylinositol 3,4,5-triphosphate in the Gi-dependent activation of platelet Rap1B. J Biol Chem. 2003;278:131–138
  27. Lova P, Paganini S, Sinigaglia F, Balduini C, Torti M. A Gi-dependent pathway is required for activation of the small GTPase RapIB in human platelets. J Biol Chem. 2002;277:12009–12015
  28. Chrzanowska-Wodnicka M, Smyth SS, Schoenwaelder SM, Fischer TH, White GC. Rap1b is required for normal platelet function and hemostasis in mice. J Clin Invest. 2005;115:680–687

 A part of this work was presented in XXth Congress of the International Society on Thrombosis and Haemostasis, August 11, 2005, Sydney, Australia.

PII: S0049-3848(05)00434-2

doi: 10.1016/j.thromres.2005.11.002

Thrombosis Research
Volume 118, Issue 4 , Pages 509-521 , 2006