A simple technique to determine thrombopoiesis level using immature platelet fraction (IPF)

Abe, Yasunori; Wada, Hideo; Tomatsu, Hiroaki; Sakaguchi, Akane; Nishioka, Junji; Yabu, Yasunori; Onishi, Katsuya; Nakatani, Kaname; Morishita, Yoshitaka; Oguni, Shinichiro; Nobori, Tsutomu

doi:10.1016/j.thromres.2005.09.007

« Previous Next »Thrombosis Research
Volume 118, Issue 4 , Pages 463-469, 2006

A simple technique to determine thrombopoiesis level using immature platelet fraction (IPF)

Yasunori Abe
- Central Clinical Laboratories, Mie University Hospital, Tsu-city, Mie, Japan
Hideo Wada
- Department of Laboratory Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu-city, Mie 514-8507, Japan
- Corresponding author. Tel.: +81 59 232 1111; fax: +81 59 231 5204.
Hiroaki Tomatsu
- Central Clinical Laboratories, Mie University Hospital, Tsu-city, Mie, Japan
Akane Sakaguchi
- Central Clinical Laboratories, Mie University Hospital, Tsu-city, Mie, Japan
Junji Nishioka
- Central Clinical Laboratories, Mie University Hospital, Tsu-city, Mie, Japan
Yasunori Yabu
- Department of Laboratory Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu-city, Mie 514-8507, Japan
Katsuya Onishi
- Department of Laboratory Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu-city, Mie 514-8507, Japan
Kaname Nakatani
- Department of Laboratory Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu-city, Mie 514-8507, Japan
Yoshitaka Morishita
- Central Clinical Laboratories, Mie University Hospital, Tsu-city, Mie, Japan
Shinichiro Oguni
- Sysmex Corporation, Product Development, Kobe, Japan
Tsutomu Nobori
- Department of Laboratory Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu-city, Mie 514-8507, Japan

Received 26 April 2005; received in revised form 27 August 2005; accepted 15 September 2005.

Abstract

Immature platelet fraction (IPF) has been measured by fully automated analyzer (XE-2100) as reticulated platelet (RP) which is reflected with thrombopoiesis in bone marrow.

IPF value in the healthy volunteers was 3.3% (1.0–10.3) and upper 95% confidential interval (95% CI) of IPF was determined as 7.7%. IPF was significantly high in the patients with idiopathic thrombocytopenic purpura (ITP; 17.4%, 1.2–53.2%) and recovery phase of post-chemotherapy, and significantly low in nadir phase of post-chemotherapy, and within normal range in the patients with ITP in complete remission (CR) and with aplastic anemia (AA). Total count of IPF was significantly low in patients with ITP, AA or post-chemotherapy. Mean platelet volume (MPV) was significantly high in only patients with ITP.

IPF 7.7% is best point for highest sensitivity (86.8%) and specificity (92.6%) in diagnosis of ITP and recovery phase of post-chemotherapy. In receiver operating characteristic curve for diagnosis of ITP and recovery phase of post-chemotherapy, IPF was significantly more useful than MPV.

These results show that IPF reflects the pathology of thrombocytopenic disorders, and that measurement of IPF is useful for the differential diagnosis and analysis of platelet kinetics.

Keywords: IPF (immature platelet fraction), Reticulated platelet, ITP (idiopathic thrombocytopenic purpura), AA (aplastic anemia)